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Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article 
Author(s):
Gaspersic, J; Hafner-Bratkovic, I; Stephan, M; Veranic, P; Bencina, M; Vorberg, I; Jerala, R 
Title:
Tetracysteine-tagged prion protein allows discrimination between the native and converted forms. 
Abstract:
The conformational conversion of prion protein (PrP) from a native conformation to the amyloid form is a hallmark of transmissible spongiform encephalopathies. Conversion is usually monitored by fluorescent dyes, which bind generic amyloids and are less suited for living cell imaging. We report a new method for the synthesis of membrane-permeable and membrane-impermeable biarsenical reagents, which are then used to monitor murine PrP (mPrP) misfolding. We introduced tetracysteine (TC) tags into...    »
 
Journal title abbreviation:
FEBS J 
Year:
2010 
Journal volume:
277 
Journal issue:
Pages contribution:
2038-50 
Language:
eng 
Print-ISSN:
1742-464X 
TUM Institution:
Institut für Virologie