Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.

Document type:: Zeitschriftenaufsatz
Author(s):: Mishra, Kanuj; Stankevych, Mariia; Fuenzalida-Werner, Juan Pablo; Grassmann, Simon; Gujrati, Vipul; Huang, Yuanhui; Klemm, Uwe; Buchholz, Veit R; Ntziachristos, Vasilis; Stiel, Andre C
Title:: Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
Abstract:: We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
Journal title:: Science Advances
Journal title abbreviation:: Sci Adv
Year:: 2020
Journal volume:: 6
Journal issue:: 24
Fulltext / DOI:: doi:10.1126/sciadv.aaz6293
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/32582850
WWW:: https://www.science.org/doi/10.1126/sciadv.aaz6293
Print-ISSN:: 2375-2548
TUM Institution:: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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