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Document type:
Zeitschriftenaufsatz 
Author(s):
Bian, Yangyang; The, Matthew; Giansanti, Piero; Mergner, Julia; Zheng, Runsheng; Wilhelm, Mathias; Boychenko, Alexander; Kuster, Bernhard 
Title:
Identification of 7 000–9 000 Proteins from Cell Lines and Tissues by Single-Shot Microflow LC–MS/MS 
Abstract:
A current trend in proteomics is to acquire data in a "single-shot" by LC-MS/MS because it simplifies workflows and promises better throughput and quantitative accuracy than schemes that involve extensive sample fractionation. However, single-shot approaches can suffer from limited proteome coverage when performed by data dependent acquisition (ssDDA) on nanoflow LC systems. For applications where sample quantities are not scarce, this study shows that high proteome coverage can be obtained usin...    »
 
Journal title:
Analytical Chemistry 
Year:
2021 
Journal volume:
93 
Journal issue:
25 
Pages contribution:
8687-8692 
Publisher:
American Chemical Society (ACS) 
E-ISSN:
0003-27001520-6882 
Date of publication:
14.06.2021