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Document type:
Journal Article
Author(s):
Probst, Florian Andreas; Fliefel, Riham; Burian, Egon; Probst, Monika; Eddicks, Matthias; Cornelsen, Matthias; Riedl, Christina; Seitz, Hermann; Aszódi, Attila; Schieker, Matthias; Otto, Sven
Title:
Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds.
Abstract:
Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixteen skeletally mature miniature pigs were divided into two groups: Control group and scaffolds seeded with osteogenic differentiated pADSCs (n = 8/group). TCP-PLGA scaffolds with or without cells were integrated in the mandibular critical size defects and fixed by titanium osteosynthesis plates. After 12 weeks, ADSCs seeded scaffolds (n = 7) demonstrated significantly higher bone volume (34.8% ± 4.80%) than scaffolds implanted without cells (n = 6, 22.4% ± 9.85%) in the micro-CT (p < 0.05). Moreover, an increased amount of osteocalcin deposition was found in the test group in comparison to the control group (27.98 ± 2.81% vs 17.10 ± 3.57%, p < 0.001). In conclusion, ADSCs seeding on ceramic/polymer scaffolds improves bone regeneration in large mandibular defects. However, further improvement with regard to the osteogenic capacity is necessary to transfer this concept into clinical use.
Journal title abbreviation:
Sci Rep
Year:
2020
Journal volume:
10
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41598-020-59038-8
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/32029875
Print-ISSN:
2045-2322
TUM Institution:
Fachgebiet Neuroradiologie (Prof. Zimmer)
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