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Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't 
Autor(en):
Romanelli, Elisa; Sorbara, Catherine D; Niki?, Ivana; Dagkalis, Athanasios; Misgeld, Thomas; Kerschensteiner, Martin 
Titel:
Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes. 
Abstract:
Here we provide a protocol for rapidly labeling different cell types, distinct subcellular compartments and key injury mediators in the spinal cord of living mice. This method is based on the application of synthetic vital dyes to the surgically exposed spinal cord. Suitable vital dyes applied in appropriate concentrations lead to reliable in vivo labeling, which can be combined with genetic tags and in many cases preserved for postfixation analysis. In combination with in vivo imaging, this app...    »
 
Zeitschriftentitel:
Nat Protoc 
Jahr:
2013 
Band / Volume:
Heft / Issue:
Seitenangaben Beitrag:
481-90 
Sprache:
eng 
Volltext / DOI:
Print-ISSN:
1754-2189 
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)