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- Document type:
- Zeitschriftenaufsatz
- Author(s):
- Steck, Natalie; Hoffmann, Micha; Sava, Irina G.; Kim, Sandra C.; Hahne, Hannes; Tonkonogy, Susan L.; Mair, Katrin; Krueger, Dagmar; Pruteanu, Mihaela; Shanahan, Fergus; Vogelmann, Roger; Schemann, Michael; Kuster, Bernhard; Sartor, R. Balfor; Haller, Dirk
- Title:
- Enterococcus faecalis metalloprotease compromises epithelial barrier and contributes to intestinal inflammation.
- Abstract:
- BACKGROUND & AIMS: Matrix metalloproteases (MMPs) mediate pathogenesis of chronic intestinal inflammation. We characterized the role of the gelatinase (GelE), a metalloprotease from Enterococcus faecalis, in the development of colitis in mice. METHODS: Germ-free, interleukin-10-deficient (IL-10(-/-)) mice were monoassociated with the colitogenic E faecalis strain OG1RF and isogenic, GelE-mutant strains. Barrier function was determined by measuring E-cadherin expression, transepithelial electrical resistance (TER), and translocation of permeability markers in colonic epithelial cells and colon segments from
- Keywords:
- Mice, Knockout, Disease Models, Animal, Animals, Mice, Cadherins/metabolism, CD4-Positive T-Lymphocytes/physiology, Cell Membrane Permeability/*physiology, Colitis/etiology/metabolism/*physiopathology, Enterococcus faecalis/*metabolism, Gelatinases/*metabolism, Gram-Positive Bacterial Infections/complications/metabolism/*physiopathology, Interleukin-10/genetics/metabolism, Intestinal Mucosa/cytology/*metabolism, Metalloproteases/*metabolism, Mice, Mutant Strains, Tumor Necrosis Factor-alpha/gene... »
- Journal title:
- Gastroenterology
- Year:
- 2011
- Journal volume:
- 141
- Month:
- sep
- Journal issue:
- 3
- Pages contribution:
- 959--971
- Language:
- eng
- Fulltext / DOI:
- doi:10.1053/j.gastro.2011.05.035
- Print-ISSN:
- 1528-0012 0016-5085
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