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- Dokumenttyp:
- Journal Article
- Autor(en):
- Mohr, Fabian; Przibilla, Sabine; Leonhardt, Franziska; Stemberger, Christian; Dreher, Stefan; Müller, Thomas R; Fräßle, Simon P; Schmidt, Georg P; Kiene, Marie-Luise; Stadler, Herbert; Busch, Dirk H
- Titel:
- Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
- Abstract:
- We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent funct... »
- Zeitschriftentitel:
- Sci Rep
- Jahr:
- 2018
- Band / Volume:
- 8
- Heft / Issue:
- 1
- Volltext / DOI:
- doi:10.1038/s41598-018-34589-z
- PubMed:
- http://view.ncbi.nlm.nih.gov/pubmed/30425259
- Print-ISSN:
- 2045-2322
- TUM Einrichtung:
- Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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