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Document type:
Zeitschriftenaufsatz 
Author(s):
Zecha, Jana; Gabriel, Wassim; Spallek, Ria; Chang, Yun-Chien; Mergner, Julia; Wilhelm, Mathias; Bassermann, Florian; Kuster, Bernhard 
Title:
Linking post-translational modifications and protein turnover by site-resolved protein turnover profiling 
Abstract:
Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labeling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ubiquitinated peptides for >9,000 native proteins. This site-resolved protein turnover (SPOT) profiling discloses global and site-specific differences in turnover associated with the presence or absenc...    »
 
Keywords:
BayBioMS; Mass spectrometry; Post-translational modifications; Proteolysis; Proteomics 
Journal title:
Nature Communications 
Year:
2022 
Journal volume:
13 
Journal issue:
Publisher:
Springer Science and Business Media LLC 
E-ISSN:
2041-1723 
Date of publication:
10.01.2022