Gegenstand dieser Dissertation ist die Klonierung der ispG- und ispH-Gene des Deoxyxylulosephosphat-Wegs aus den Pflanzen Solanum lycopersicum und Zea mays, sowie die biochemische Charakterisierung der rekombinanten IspG-Proteine aus den Bakterien Aquifex aeolicus, Bacillus subtilis, Haemophilus influenzae und Mycobacterium tuberculosis und des rekombinanten IspH-Proteins aus Thermotoga maritima. Zudem wurde mit dem IspG-Protein aus H. influenzae und dem IspH-Protein aus T. maritima 2C-Methyl-D-erythritol-2,4-cyclodiphosphat in größeren Maßstäben zu 1-Hydroxy-2-methyl-2-(E)-butenyl-4-diphosphat bzw. zu Isopentenyldiphosphat/Dimethylallyldiphosphat enzymatische katalysiert.     «

Gegenstand dieser Dissertation ist die Klonierung der ispG- und ispH-Gene des Deoxyxylulosephosphat-Wegs aus den Pflanzen Solanum lycopersicum und Zea mays, sowie die biochemische Charakterisierung der rekombinanten IspG-Proteine aus den Bakterien Aquifex aeolicus, Bacillus subtilis, Haemophilus influenzae und Mycobacterium tuberculosis und des rekombinanten IspH-Proteins aus Thermotoga maritima. Zudem wurde mit dem IspG-Protein aus H. influenzae und dem IspH-Protein aus T. maritima 2C-Methyl-D-...     »

The subject of this PhD thesis is the cloning of genes ispG and ispH of the deoxyxylulose phosphate pathway from the plants Solanum lycopersicum and Zea mays as well as the biochemical characterization of recombinant IspG proteins from Aquifex aeolicus, Bacillus subtilis, Haemophilus influenzae and Mycobacterium tuberculosis and of the recombinant IspH protein from Thermotoga maritima. The IspG protein from H. Influenzae and the IspH protein from T maritima were used to synthesise (E)-4-hydroxy-3-methylbut-2-enyl diphosphate and isopentenyl diphosphate/dimethylallyl diphosphate, respectively, from 2-C-methyl-D-erythritol-2,4-cyclodiphosphate in high amounts to use as substrate.     «

The subject of this PhD thesis is the cloning of genes ispG and ispH of the deoxyxylulose phosphate pathway from the plants Solanum lycopersicum and Zea mays as well as the biochemical characterization of recombinant IspG proteins from Aquifex aeolicus, Bacillus subtilis, Haemophilus influenzae and Mycobacterium tuberculosis and of the recombinant IspH protein from Thermotoga maritima. The IspG protein from H. Influenzae and the IspH protein from T maritima were used to synthesise (E)-4-hydroxy-...     »