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Document type:
Zeitschriftenaufsatz 
Author(s):
Bottini, M.; Venere, A.; Lugli, P.; Rosato, N. 
Title:
Conformation and stability of myoglobin in dilute and crowded organically modified media 
Abstract:
In this study we investigated the structural features of myoglobin, a heme-containing enzyme, upon encapsulation in tetramethoxysilane and organically modified sol–gel glasses. Absorption and fluorescence spectroscopy have been employed to study the conformational changes of the protein embedded in these solid matrices. The slight decrease of the heme absorption value during gelation in all the different glasses indicates an irreversible change in the heme molar absorptivity consistent with the...    »
 
Keywords:
Myoglobin, Mb; Tryptophan, TRP; Guanidinium hydrochloride, GdHCl; Organically modified silica gels, ORMOSIL; Tetramethyl-orthosilicate, TMOS; 3-Aminopropyl-trimethoxysilane, APTS; 3-Glycidyloxypropyl-trimethoxysilane, GLYMO; 3-Trimethoxysilyl-propyl methacrylate, TMSPM 
Journal title:
Journal of Non-Crystalline Solids Volume 343, Issues 1–3, 1 September 2004, Pages 101–108 
Year:
2004 
Year / month:
2004-09 
Quarter:
3. Quartal 
Month:
Sep 
Pages contribution:
101 - 108 
Language:
en 
Publisher:
Elsevier B.V.