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Dokumenttyp:
journal article 
Autor(en):
Dorsch, S; Klotz, KN; Engelhardt, S; Lohse, MJ; Bünemann, M 
Titel:
Analysis of receptor oligomerization by FRAP microscopy. 
Abstract:
Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP. We validated this approach with CD86 and CD28 as monomeric and dimeric reference proteins, respectively. Di- or oligomerization of G protein-coupled receptors is strongly debated. We studied human...    »
 
Zeitschriftentitel:
Nat Methods 
Jahr:
2009 
Band / Volume:
Heft / Issue:
Seitenangaben Beitrag:
225-30 
Sprache:
eng 
Print-ISSN:
1548-7091 
TUM Einrichtung:
r Pharmakologie und Toxikologie