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Dokumenttyp:
journal article 
Autor(en):
Lin, DH; Yue, P; Pan, CY; Sun, P; Zhang, X; Han, Z; Roos, M; Caplan, M; Giebisch, G; Wang, WH 
Titel:
POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels. 
Abstract:
POSH (plenty of SH3) is a scaffold protein that has been shown to act as an E3 ubiquitin ligase. Here we report that POSH stimulates the ubiquitination of Kir1.1 (ROMK) and enhances the internalization of this potassium channel. Immunostaining reveals the expression of POSH in the renal cortical collecting duct. Immunoprecipitation of renal tissue lysate with ROMK antibody and glutathione S-transferase pulldown experiments demonstrated the association between ROMK and POSH. Moreover, immunopreci...    »
 
Zeitschriftentitel:
J Biol Chem 
Jahr:
2009 
Band / Volume:
284 
Heft / Issue:
43 
Seitenangaben Beitrag:
29614-24 
Sprache:
eng 
Print-ISSN:
0021-9258 
TUM Einrichtung:
r Nephrologie