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Document type:
Zeitschriftenaufsatz 
Author(s):
Wiedemann, S.; Lutz, B.; Albrecht, C.; Kuehn, R.; Killermann, B.; Einspanier, R.; Meyer, H.H.D 
Title:
Fate of genetically modified maize and conventional rapeseed, and endozoochory in wild boar (Sus scrofa
Abstract:
Feeding experiments were carried out to investigate the digestive fate of transgenic DNA and novel protein in wild boar applying polymerase chain reaction (PCR) and immunodiagnostic techniques. Furthermore, the dispersal of viable maize and rapeseed (endozoochory) was studied. A diet containing conventional rapeseed, and either genetically modified (GM) maize expressing Cry1Ab protein (Bt176) or non-GM isogenic maize was offered. By conventional and quantitative PCR both chloroplast-specific plant DNA (rubisco) and Cry1Ab gene fragments were detected only in gastrointestinal content. Using an enzyme-linked immunosorbent assay (ELISA) positive signals of immunoactive Cry1Ab protein were detected in digesta samples. Analysis of endozoochory showed that excreted maize seeds retain their germination capacity only in extremely rare cases and no intact rapeseed was found in faeces. A possible dispersal of viable seeds by wild boars is higly unlikely. 
Keywords:
Wild boar, Bt-maize, Endozoochory, Recombinant DNA, Cry1Ab protein 
Journal title:
Mammalian Biology 
Year:
2009 
Journal volume:
74 
Pages contribution:
191-197 
Language:
en 
TUM Institution:
Zentralinstitut für Ernährungs- und Lebensmittelforschung / Abteilung Physiologie 
Format:
Text