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Document type:
Zeitschriftenaufsatz 
Author(s):
Reiter, M.; Pfaffl, M.W 
Title:
Effects of plate position, plate type and sealing systems on real-time PCR results 
Abstract:
Real-time PCR has become an important tool for quantitative nucleic acids analysis. By now, a wide rang of real-time PCR instruments, PCR plastic consumables and sealing systems are available. In this study both transparent and white plates were taken to test possible position and plate effects on quantitative PCR results on different real-time platforms: ep realplex (Eppendorf, Hamburg, Germany) and the iQ5 (Bio-Rad, Palo Alto, USA). Heat and adhesive sealing was used to compare sealing sys...    »
 
Keywords:
real-time qRT-PCR, position effects, plate type, sealing system 
Journal title:
Biotechnology & Biotechnological Equipment 
Year:
2008 
Journal volume:
22: 
Journal issue:
(3) 
Pages contribution:
824-828 
Language:
en 
TUM Institution:
Zentralinstitut für Ernährungs- und Lebensmittelforschung / Abteilung Physiologie 
Format:
Text