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- Dokumenttyp:
- Zeitschriftenaufsatz
- Autor(en):
- Kaczmarek, M.M.; Waclawik, A.; Blitek, A.; Kowalczyk, A.E.; Schams, D.; Ziecik, A.J
- Titel:
- Expression of the vascular endothelial growth factor-receptor system in the porcine endometrium throughout the estrous cycle and early pregnancy
- Abstract:
- The objective of this study was to investigate the protein and RNA expression of vascular endothelial growth factor (VEGF), VEGFR-1 (fms-like tyrosine kinase, Flt-1) and VEGFR-2 (fetal liver kinase-1/kinase insert domain-containing receptor, Flk-1/KDR) in the endometrium during the estrous cycle and early pregnancy in pigs. The VEGF-receptor system was localized in epithelial and stromal cells, blood vessels, and myometrium. Western blot analysis showed higher levels of VEGF protein during the periovulatory and periimplantation periods (P<0.001, and P<0.05, respectively). Constant expression of VEGF mRNA during the cycle and significant upregulation on Days 22–25 of gestation (vs. Days 9–17; P<0.001) was observed. Stable levels of VEGFR-1 mRNA and protein were detected in the endometrium of cyclic animals. However, higher VEGFR-1 protein expression was found on Days 16–17 of the estrous cycle (P<0.01) and Days 13–15 of gestation (P<0.05). Protein expression of VEGFR-2 was elevated on Days 2–4 of the estrous cycle (P<0.001), but mRNA levels were constant during the cycle. In pregnancy, VEGFR-2 protein expression started to increase after Day 15 (vs. Days 9–12; P<0.05), but induction of VEGFR-2 mRNA expression occurred earlier on Days 13–15. It appears from the present study that the VEGF-receptor system is regulated in a temporal and spatial manner during the estrous cycle and early pregnancy in pigs. The results suggest that VEGF-A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs.
- Stichworte:
- angiogenesis; VEGF; VEGFR-1; VEGFR-2; endometrium; pig
- Zeitschriftentitel:
- Molecular Reproduction and Development
- Jahr:
- 2008
- Band / Volume:
- 75:
- Seitenangaben Beitrag:
- 362-372
- Sprache:
- en
- Volltext / DOI:
- doi:10.1002/mrd.20815
- WWW:
- interscience.wiley.com
- Angenommen (von Zeitschrift):
- 22.06.2007
- Publikationsdatum:
- 14.09.2007
- TUM Einrichtung:
- Zentralinstitut für Ernährungs- und Lebensmittelforschung / Abteilung Physiologie
- Format:
- Text
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