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Document type:
journal article 
Author(s):
Dölken, L; Ruzsics, Z; Rädle, B; Friedel, CC; Zimmer, R; Mages, J; Hoffmann, R; Dickinson, P; Forster, T; Ghazal, P; Koszinowski, UH 
Title:
High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay. 
Abstract:
RNA levels in a cell are determined by the relative rates of RNA synthesis and decay. State-of-the-art transcriptional analyses only employ total cellular RNA. Therefore, changes in RNA levels cannot be attributed to RNA synthesis or decay, and temporal resolution is poor. Recently, it was reported that newly transcribed RNA can be biosynthetically labeled for 1-2 h using thiolated nucleosides, purified from total cellular RNA and subjected to microarray analysis. However, in order to study sign...    »
 
Journal title abbreviation:
RNA 
Year:
2008 
Journal volume:
14 
Journal issue:
Pages contribution:
1959-72 
Language:
eng 
Print-ISSN:
1355-8382 
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene