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Dokumenttyp:
journal article 
Autor(en):
Zakariassen, H; Cederkvist, FH; Harbitz, E; Shimizu, T; Lange, R; Mayer, B; Gorren, AC; Andersson, KK; Sørlie, M 
Titel:
Thermodynamic analysis of L-arginine and N omega-hydroxy-L-arginine binding to nitric oxide synthase. 
Abstract:
Isothermal titration calorimetry has been used to determine thermodynamic parameters of substrate binding to the oxygenase domain of neuronal nitric oxide synthase (nNOS(oxy)) in the presence of the cofactor tetrahydrobiopterin. The intermediate N(omega)-hydroxy-L-arginine (NHA) has a larger affinity than L-Arginine (L-Arg) for nNOS(oxy), with K(d)=0.4+/-0.1 microM and 1.7+/-0.3 microM at 25 degrees C, respectively. nNOS(oxy) binds NHA and L-Arg with DeltaH -4.1+/-0.2 and -1.0+/-0.1 kcal/mol and...    »
 
Zeitschriftentitel:
Biochim Biophys Acta 
Jahr:
2008 
Band / Volume:
1784 
Heft / Issue:
Seitenangaben Beitrag:
806-10 
Sprache:
eng 
Print-ISSN:
0006-3002 
TUM Einrichtung:
chirurgie