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Document type:
journal article 
Author(s):
Zakariassen, H; Cederkvist, FH; Harbitz, E; Shimizu, T; Lange, R; Mayer, B; Gorren, AC; Andersson, KK; Sørlie, M 
Title:
Thermodynamic analysis of L-arginine and N omega-hydroxy-L-arginine binding to nitric oxide synthase. 
Abstract:
Isothermal titration calorimetry has been used to determine thermodynamic parameters of substrate binding to the oxygenase domain of neuronal nitric oxide synthase (nNOS(oxy)) in the presence of the cofactor tetrahydrobiopterin. The intermediate N(omega)-hydroxy-L-arginine (NHA) has a larger affinity than L-Arginine (L-Arg) for nNOS(oxy), with K(d)=0.4+/-0.1 microM and 1.7+/-0.3 microM at 25 degrees C, respectively. nNOS(oxy) binds NHA and L-Arg with DeltaH -4.1+/-0.2 and -1.0+/-0.1 kcal/mol and...    »
 
Journal title abbreviation:
Biochim Biophys Acta 
Year:
2008 
Journal volume:
1784 
Journal issue:
Pages contribution:
806-10 
Language:
eng 
Print-ISSN:
0006-3002 
TUM Institution:
chirurgie