Nitric oxide inhibition and consecutive Aspisol application show a prolonged survival of orthotopic transplanted livers in a rat model.

Matevossian, E; Hüser, N; Kern, H; Assfalg, V; Preissel, A; Sinicina, I; Stangl, M; Thorban, S

doi:10.1016/j.transproceed.2008.03.041

journal article

Dokumenttyp:: Journal Article; Proceedings Paper
Autor(en):: Matevossian, E; Hüser, N; Kern, H; Assfalg, V; Preissel, A; Sinicina, I; Stangl, M; Thorban, S
Titel:: Nitric oxide inhibition and consecutive Aspisol application show a prolonged survival of orthotopic transplanted livers in a rat model.
Abstract:: BACKGROUND: It is generally accepted that nitric oxide (NO) plays a crucial role in acute rejection caused by inflammatory responses. Therefore, the purpose of this study was to investigate the effect on survival following arterialized orthotopic rat liver transplantations (o-RLTx) of NO inhibition and consequent blockade of platelet aggregation by application of Aspisol. MATERIALS AND METHODS: Inbred LEWIS-(RT(1)) rats underwent arterialized o-RLTx under ether anesthesia with DA-(RT1av1) rats as organ donors. After liver transplantation, serum parameters were determined and hepatic biopsy specimens were sampled on postoperative days 5, 8, 10, 30, and 90. Sixty-one rats were divided into 5 groups: syngenic controls (group I, n = 12); allogenic controls (group II, n = 11); allogenic with FK506 immunosuppression (group III, n = 12); allogenic with AGH-treatment (group IV, n = 13); and allogenic with AGH/low- dose Aspisol treatment for 5 days after liver transplantation (group V, n = 13) (Bayer, Leverkusen, Germany). RESULTS: Rats of group V with AGH/low-dose Aspisol treatment showed significantly longer graft survival (18.2 days +/- 1.8 days) compared with group II rats with untreated grafts (11.3 days +/- 1.7 days) the allogenic group IV with AGH treatment (11.2 days +/- 1.8 days; P < .05). Histological examination revealed moderate graft rejection among the AGH-treated group IV; however, marked platelet aggregation in sinusoids was present, which was not observed in the AGH/low-dose Aspisol-treated animals (group V). CONCLUSION: Our data suggested that simultaneous treatment with AGH/low-dose Aspisol leads to a significant increase in survival and inhibition of platelet aggregation in the graft after orthotopic liver transplantation.
Zeitschriftentitel:: Transplant Proc
Jahr:: 2008
Band / Volume:: 40
Heft / Issue:: 4
Seitenangaben Beitrag:: 971-3
Sprache:: eng
Volltext / DOI:: doi:10.1016/j.transproceed.2008.03.041
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/18555092
Print-ISSN:: 0041-1345
TUM Einrichtung:: Chirurgische Klinik und Poliklinik
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Chirurgie (Prof. Friess)2008