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Document type:
journal article 
Author(s):
Zischka, H; Lichtmannegger, J; Jägemann, N; Jennen, L; Hamöller, D; Huber, E; Walch, A; Summer, KH; Göttlicher, M 
Title:
Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free flow device (ZE-FFE). 
Abstract:
This protocol describes the purification of mitochondria from rat liver with the aid of zone electrophoresis in a free flow device (ZE-FFE). Starting from liver homogenate, cell debris and nuclei are removed by low speed centrifugation. A crude mitochondrial fraction is obtained by medium speed centrifugation and is further purified by washing followed by a Nycodenz gradient centrifugation. Lysosomes and microsomes are located at the upper parts of the gradient, whereas mitochondria are found in...    »
 
Journal title abbreviation:
Methods Mol Biol 
Year:
2008 
Journal volume:
424 
Pages contribution:
333-48 
Language:
eng 
Print-ISSN:
1064-3745 
TUM Institution:
Institut für Toxikologie und Umwelthygiene