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- Document type:
- Journal Article; Research Support, Non-U.S. Gov't
- Author(s):
- Fuchs, AT; Kuehnl, A; Pelisek, J; Rolland, PH; Mekkaoui, C; Netz, H; Nikol, S
- Title:
- Inhibition of restenosis formation without compromising reendothelialization as a potential solution to thrombosis following angioplasty?
- Abstract:
- Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p<.05), but no suppression of endothelial growth using CNP. Applied in vivo, angiography revealed a trend of reduced restenosis formation in balloon-injured porcine arteries treated with CNP gene or beta-galactosidase (beta-Gal) control gene after three months (2.59 +/- 2.04-fold reduction, p = n.s.). Histologically, morphometry revealed significantly reduced neointima formation after treatment with CNP plasmid (7.26 +/- 1.44-fold reduction, p < .05). Evans blue staining demonstrated complete endothelial repair already 3 weeks after intervention using CNP. Transfer of CNP gene resulted in a significant inhibition of neointima formation without compromising endothelial repair. Therefore, use of the CNP gene may offer a solution to suppress restenosis formation while preventing subacute or late thrombosis.
- Journal title abbreviation:
- Endothelium
- Year:
- 2008
- Journal volume:
- 15
- Journal issue:
- 1
- Pages contribution:
- 85-92
- Language:
- eng
- Fulltext / DOI:
- doi:10.1080/10623320802092484
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/18568948
- Print-ISSN:
- 1062-3329
- TUM Institution:
- Fachgebiet Gefäßchirurgie (Prof. Eckstein)
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