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Document type:
journal article 
Author(s):
Fuchs, AT; Kuehnl, A; Pelisek, J; Rolland, PH; Mekkaoui, C; Netz, H; Nikol, S 
Title:
Inhibition of restenosis formation without compromising reendothelialization as a potential solution to thrombosis following angioplasty? 
Abstract:
Stent thrombosis remains an important problem after the implantation of different stent types. A potential solution to this problem may be vasoactive agents with dual effects on different cell types like C-type natriuretic peptide (CNP). Therefore, in vitro and in vivo effects of CNP were investigated in a porcine restenotic model. Gene transfer of CNP in cultures of porcine vascular cells revealed up to 30% reduction of growth of smooth muscle cells (p<.05), but no suppression of endothelial growth using CNP. Applied in vivo, angiography revealed a trend of reduced restenosis formation in balloon-injured porcine arteries treated with CNP gene or beta-galactosidase (beta-Gal) control gene after three months (2.59 +/- 2.04-fold reduction, p = n.s.). Histologically, morphometry revealed significantly reduced neointima formation after treatment with CNP plasmid (7.26 +/- 1.44-fold reduction, p< .05). Evans blue staining demonstrated complete endothelial repair already 3 weeks after intervention using CNP. Transfer of CNP gene resulted in a significant inhibition of neointima formation without compromising endothelial repair. Therefore, use of the CNP gene may offer a solution to suppress restenosis formation while preventing subacute or late thrombosis. 
Journal title abbreviation:
Endothelium 
Year:
2008 
Journal volume:
15 
Journal issue:
Pages contribution:
85-92 
Language:
eng 
Print-ISSN:
1062-3329 
TUM Institution:
chirurgie