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- Document type:
- Journal Article; Article
- Author(s):
- Henning, T; Weber, AW; Bauer, JS; Meier, R; Carlsen, JM; Sutton, EJ; Prevrhal, S; Ziegler, SI; Feussner, H; Daldrup-Link, HE; Rummeny, EJ
- Title:
- Imaging Characteristics of DHOG, a Hepatobiliary Contrast Agent for Preclinical MicroCT in Mice.
- Abstract:
- RATIONALE AND OBJECTIVES: This study was performed to assess the imaging characteristics and pharmacokinetics of 1,3-Bis-[7-(3-amino-2,4,6-triiodophenyl)-heptanoyl]-2-oleoyl glycerol (DHOG, Fenestra LC), a hepatobiliary contrast agent for microCT. MATERIALS AND METHODS: We investigated the abdomen of 18 female C3H mice in a MicroCAT II microCT scanner before contrast agent injection and at multiple time points up to 48 hours after intravenous injection of DHOG (1 g I/kg body weight). The contrast agent effect was determined quantitatively and dynamically by measuring pre- and postcontrast Hounsfield units (HU) of the liver, aorta, spleen, and kidneys. Based on additional phantom measurements, the reproducibility of lesion detection was estimated for different lesion sizes. RESULTS: DHOG caused a marked early postcontrast enhancement of blood in the aorta and a very high enhancement of the spleen, both slowly declined after 90 minutes. The liver parenchyma showed a slow contrast agent accumulation and clearly increased HU data between 3 and 7 hours after injection. No significant renal parenchymal enhancement or excretion was noticed. At early time points after administration, DHOG exhibits characteristics of a macromolecular contrast agent by demonstrating a blood pool effect. At later time points, DHOG provides a prolonged, marked liver enhancement on microCT images due to its specific liver uptake. For a lesion size of 1 mm diameter, the variability in between two scans was 27.7 HU (P < .05) and the variability for different planes of one scan was 19.8 HU (P < .05). CONCLUSIONS: DHOG yields a very good visualization of the liver and delineation of the surrounding structures with a long plateau. It is a very suitable contrast agent for liver imaging in mice for microCT imaging. The presented protocol provides a high reproducibility for lesion detection with a relatively low radiation dose.
- Journal title abbreviation:
- Acad Radiol
- Year:
- 2008
- Journal volume:
- 15
- Journal issue:
- 3
- Pages contribution:
- 342-9
- Language:
- eng
- Fulltext / DOI:
- doi:10.1016/j.acra.2007.10.007
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/18280932
- Print-ISSN:
- 1076-6332
- TUM Institution:
- Chirurgische Klinik und Poliklinik; Klinik und Poliklinik für Nuklearmedizin
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