Method comparison of tumor markers assessed by LOCI (TM) - and ECLIA-based technologies

Dolscheid-Pommerich, RC; Dolscheid, S; Eichhorn, L; Zur, B; Holdenrieder, S; Stoffel-Wagner, B

Institut für Laboratoriumsmedizin (DHM) (Prof. Holdenrieder)

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Document type:: Article
Author(s):: Dolscheid-Pommerich, RC; Dolscheid, S; Eichhorn, L; Zur, B; Holdenrieder, S; Stoffel-Wagner, B
Title:: Method comparison of tumor markers assessed by LOCI (TM) - and ECLIA-based technologies
Abstract:: Background: Since the introduction of luminescent oxygen channeling immunoassays (LOCI (TM))- based assays in the daily laboratory routine of tumor marker measurements, only a small number of method comparisons with established immunoassays have been published. We performed a method comparison between LOCI (TM) - based tumor marker assays for Dimension (TM) VISTA and electrochemiluminiscent immunoassays (ECLIA) for Cobas (TM) e411, for alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), CA 125, CA 15- 3, CA 19-9, prostate-specific antigen (PSA) and free PSA (fPSA). Methods: Tumor markers were assessed in 1088 sera from routine diagnostics on the Dimension (TM) VISTA 1500 and Cobas (TM) e411 analyzers. Results: Strong correlations were achieved for PSA ( r = 0.999), AFP ( r = 0.994) and CEA ( r = 0.993). Results were quite comparable as only minor slopes of 1.05 ( PSA), 1.02 AFP) and 0.94 ( CEA), respectively, were found. However, correlations for CA 125 ( r = 0.976), CA 19- 9 ( r = 0.960), fPSA ( r = 0.950) and CA 15- 3 ( r = 0.940) were only moderate, and considerable slopes were observed for these markers with higher values for CA 19- 9 ( slope 1.50) and lower ones for CA 15- 3 ( 0.76), fPSA ( 0.75) and CA 125 ( 0.64), for Dimension (TM) VISTA 1500. Conclusions: We found excellent correlations and comparable values for AFP, CEA and PSA, but only moderate correlations for fPSA, CA 125, CA 15- 3 and CA 19- 9. The slopes for CA 19- 9, CA 15- 3, fPSA and CA 125 have to be considered when analysis methods for tumor markers are changed. «
Background: Since the introduction of luminescent oxygen channeling immunoassays (LOCI (TM))- based assays in the daily laboratory routine of tumor marker measurements, only a small number of method comparisons with established immunoassays have been published. We performed a method comparison between LOCI (TM) - based tumor marker assays for Dimension (TM) VISTA and electrochemiluminiscent immunoassays (ECLIA) for Cobas (TM) e411, for alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), CA... »
Journal title abbreviation:: J Lab Clin Med
Year:: 2017
Journal volume:: 41
Journal issue:: 1
Pages contribution:: 3-11
Language:: eng
Print-ISSN:: 0022-2143
TUM Institution:: Institut für Laboratoriumsmedizin (keine SAP-Zuordnung!)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Lehr- und Forschungskooperationen mit den Kliniken und Instituten am Deutschen Herzzentrum Institut für Laboratoriumsmedizin (DHM) (Prof. Holdenrieder)2017