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Document type:
journal article 
Author(s):
Schiwon, Maren; Ehrke-Schulz, Eric; Oswald, Andreas; Bergmann, Thorsten; Michler, Thomas; Protzer, Ulrike; Ehrhardt, Anja 
Title:
One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors. 
Abstract:
High-capacity adenoviral vectors (HCAdVs) devoid of all coding genes are powerful tools to deliver large DNA cargos into cells. Here HCAdVs were designed to deliver a multiplexed complete CRISPR/Cas9 nuclease system or a complete pair of transcription activator-like effector nucleases (TALENs) directed against the hepatitis B virus (HBV) genome. HBV, which remains a serious global health burden, forms covalently closed circular DNA (cccDNA) as a persistent DNA species in infected cells. This ccc...    »
 
Journal title abbreviation:
Mol Ther Nucleic Acids 
Year:
2018 
Journal volume:
12 
Pages contribution:
242-253 
Language:
eng 
TUM Institution:
Institut für Virologie