In order to understand and control fuel-driven self-assembly, analytic tools are needed. By applying a quenching chemistry which stops activation and deactivation reaction, a handy tool is proposed to extract the kinetic parameters of carbodiimide-driven self-assembly. The experimental workflow of creating fuel-driven supramolecular structures is demonstrated by dynamic colloids and fibers. Furthermore, the non-linear reaction kinetics are exploited to create molecular memory.