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Original title:
Characterisation of the functional role of the inducible transcription factor nuclear factor kappa-B (NF-kB) in isolated rat parietal cells 
Translated title:
Charakterisierung der Funktion des induzierbaren Transkriptionsfaktors Nuclear factor kappa-B (NF-kB) in isolierten Parietalzellen 
Year:
2003 
Document type:
Dissertation 
Institution:
Fakultät für Medizin 
Advisor:
Schepp, W. (apl. Prof. Dr.) 
Referee:
Holzmann, Bernhard (Prof. Dr.); Neumeier, Dieter (Prof. Dr.) 
Format:
Text 
Language:
en 
Subject group:
MED Medizin 
Keywords:
NF-kB; NF-kappaB; nuclear factor kappa-B; parietal cells; transkription factor; gastric; gastric acid; gastritis; Helicobacter pylori; cytokine; inflammation; TNF; TNF-a; TNF-alfa; TNF-alpha; IL-1b; IL-1beta; NF-kB1; NF-kB2; NF-kB p65; p65/p50; NF-kb p50; NF-kB p52; NF-kappaB p52; NF-kB subunits; NF-kappaB subunits; EMSA; electrophoretic mobility shift assay; gel shift; gel retardation assay; nuclear extract; nucleus; PhosphorImager; gastric epithelial cells; antisense; antisense oligonucleotide...    »
 
Translated keywords:
NF-kB; NF-kappaB; nuclear factor kappa-B; Parietalzellen; Transkriptionsfaktor; Magensäure; Gastritis; Helicobacter pylori; Zytokine; Inflammation; Entzündung; TNF; TNF-a; TNF-alfa; TNF-alpha; IL-1b; IL-1beta; NF-kB1; NF-kB2; NF-kB p65; p65/p50; NF-kB p50; NF-kB p52; NF-kappab p52; NF-kB Untereinheiten; NF-kappaB Untereinheiten; NF-kB subunits; NF-kappaB subunits; EMSA; electrophoretic mobility shift assay; gel shift; Gelshift; Retardationsassay; Kernextrakt; nukleärer Extrakt; Zellkern; Phospho...    »
 
TUM classification:
MED 430d 
Abstract:
The influence of TNF-alpha, IL-1beta or H2O2 on the activation of the inducible transcription factor NF-kappaB was studied in isolated rat parietal cells. We found that these cells express the TNF-alpha receptor TNFR1. NF-kappaB was present, and was activated by exposure of cells to H2O2 (250µM caused a 2.2-fold increase within 5min), TNF-alpha (10ng/ml; 1.8-fold in 30min) or IL-1beta (10ng/ml; 2.6-fold in 60min) in a time- and concentration dependent manner. The NF-kappaB subunits p65 and p50...    »
 
Translated abstract:
In isolierten Parietalzellen der Ratte wurde der Einfluss von TNF-alpha, IL-1beta oder H2O2 auf den induzierbaren Transkriptionsfaktor NF-kappaB untersucht. Die Expression des TNF-alpha-Rezeptors TNFR1 wurde nachgewiesen. NF-kappaB war vorhanden und wurde zeit- und konzentrationsabhängig aktiviert durch Stimulation der Zellen mit H2O2 (bei 250µM 2,2-fache Zunahme nach 5min), TNF-alpha (10ng/ml; 1,8-fach; 30min) oder IL-1beta (10ng/ml; 2,6-fach; 60min). Die NF-kappaB-Untereinheiten p65 und p5...    »
 
Publication :
Universitätsbibliothek der TU München 
Oral examination:
12.02.2003 
File size:
1261335 bytes 
Pages:
116 
Last change:
27.06.2005