User: Guest  Login
Original title:
Simultaner Nachweis von Phosphatasen und Bisphosphonaten im humanen Knochengewebe 
Translated title:
Simultaneous determination of phosphatases and bisphosphonates in human bone tissue 
Year:
2003 
Document type:
Dissertation 
Institution:
Fakultät für Medizin 
Advisor:
Stemberger, A. (apl. Prof. Dr.) 
Referee:
Stemberger, A. (apl. Prof. Dr.); Pontz, B. (Univ.-Prof. Dr. Dr.) 
Format:
Text 
Language:
de 
Subject group:
MED Medizin 
Keywords:
Bisphosphonate; Osteoklasten; Knochen; TRAP 5b; Phosphatasen; Osteoarthrose 
Translated keywords:
Bisphosphonates; osteoclast; bone; TRAP 5b; phosphatases; osteoarthritis 
Abstract:
Bisphosphonate hemmen den Stoffwechsel der Osteoklasten. Es wurde eine neue Methode entwickelt, um die tartratresistente, saure Phosphatase 5b (TRAP 5b), als Marker der Osteoklastenaktivität in humanen Knochen zu lokalisieren und zu quantifizieren. Patienten (n=12) der Kontroll- und Bisphosphonatgruppe (10mg Alendronat/d, 50d prä-OP) wurde der Hüftkopf durch eine Totalendoprothese ersetzt. Es wurden Proben aus der Kopf- (Z1), der Schenkelhals- (Z2), sowie der intertrochanteren Zone (Z3) entnomme...    »
 
Translated abstract:
Bisphosphonates inhibit osteoclast activity and induce apoptosis. A method was developed to quantify and localize tartratresistent acid phosphatase as a marker of osteoclast activity. Patients (n=12) of the control- and the bisphosphonate group (10mg alendronate/d, 50d prae-OP) underwent a total joint replacement of the hip. Samples of the femoral head area (A1), the femoral neck area- (A2) and the intertrochanteric area (A3) were harvested, grinded and TRAP 5b was analysed with an ELISA or were embedded in MMA, stained and the TRAP 5b-positive cells were counted. Bisphosphonate treatment leads to a moderate decrease in enzyme activity compared to the controls: 18% in area A3 (p<0.05), 5% in A2 and 11% in A1. Bisphosphonates may be incorporated and effective even in degenerative bone tissue. They might be able to inhibit osteolytic and degenerative processes in arthritic bone. 
Publication :
Universitätsbibliothek der TU München 
Oral examination:
02.12.2003 
File size:
1868821 bytes 
Pages:
91 
Last change:
27.06.2005