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Document type:
Journal Article 
Author(s):
Kislinger, Georg; Gnägi, Helmut; Kerschensteiner, Martin; Simons, Mikael; Misgeld, Thomas; Schifferer, Martina 
Title:
Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution. 
Abstract:
Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling approaches (FIB-SEM) yield isotropic voxels but are restricted in volume size. Now we present a hybrid method, named ATUM-FIB, which combines the advantages of both approaches. ATUM-FIB is based on serial sectioning of tissue into "semithick" (2-10 μm) sectio...    »
 
Journal title abbreviation:
iScience 
Year:
2020 
Journal volume:
23 
Journal issue:
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)