Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.

Dokumenttyp:: Zeitschriftenaufsatz
Autor(en):: Mishra, Kanuj; Stankevych, Mariia; Fuenzalida-Werner, Juan Pablo; Grassmann, Simon; Gujrati, Vipul; Huang, Yuanhui; Klemm, Uwe; Buchholz, Veit R; Ntziachristos, Vasilis; Stiel, Andre C
Titel:: Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
Abstract:: We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
Zeitschriftentitel:: Science Advances
Zeitschriftentitel:: Sci Adv
Jahr:: 2020
Band / Volume:: 6
Heft / Issue:: 24
Volltext / DOI:: doi:10.1126/sciadv.aaz6293
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/32582850
WWW:: https://www.science.org/doi/10.1126/sciadv.aaz6293
Print-ISSN:: 2375-2548
TUM Einrichtung:: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
BibTeX