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Document type:
Journal Article 
Author(s):
Barykina, Natalia V; Sotskov, Vladimir P; Gruzdeva, Anna M; Wu, You Kure; Portugues, Ruben; Subach, Oksana M; Chefanova, Elizaveta S; Plusnin, Viktor V; Ivashkina, Olga I; Anokhin, Konstantin V; Vlaskina, Anna V; Korzhenevskiy, Dmitry A; Nikolaeva, Alena Y; Boyko, Konstantin M; Rakitina, Tatiana V; Varizhuk, Anna M; Pozmogova, Galina E; Subach, Fedor V 
Title:
FGCaMP7, an Improved Version of Fungi-Based Ratiometric Calcium Indicator for In Vivo Visualization of Neuronal Activity. 
Abstract:
Genetically encoded calcium indicators (GECIs) have become a widespread tool for the visualization of neuronal activity. As compared to popular GCaMP GECIs, the FGCaMP indicator benefits from calmodulin and M13-peptide from the fungi Aspergillus niger and Aspergillus fumigatus, which prevent its interaction with the intracellular environment. However, FGCaMP exhibits a two-phase fluorescence behavior with the variation of calcium ion concentration, has moderate sensitivity in neurons (as compare...    »
 
Journal title abbreviation:
Int J Mol Sci 
Year:
2020 
Journal volume:
21 
Journal issue:
Fulltext / DOI:
Print-ISSN:
1422-0067 
TUM Institution:
Institut für Neurowissenschaften