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Document type:
Journal Article 
Author(s):
Kabiri, Yaschar; von Toerne, Christine; Fontes, Adriana; Knolle, Percy A; Zischka, Hans 
Title:
Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses. 
Abstract:
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable method to isolate high microgram to low milligram amounts of intact mitochondria from cell culture samples along with an optional density gradient puri...    »
 
Journal title abbreviation:
Methods Mol Biol 
Year:
2021 
Journal volume:
2261 
Pages contribution:
411-419 
Print-ISSN:
1064-3745 
TUM Institution:
Institut für Toxikologie und Umwelthygiene