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Document type:
Journal Article 
Author(s):
Marinkovi?, Petar; Godinho, Leanne; Misgeld, Thomas 
Title:
Generation of Thy1 Constructs for Pronuclear Injection. 
Abstract:
With easy access to core facilities or commercial providers of pronuclear injections, generating simple Thy1-XFP transgenic mice (where XFP stands for any fluorescent protein) is now a possibility even for small laboratories. The generation of new Thy1 transgenic lines generally consists of five steps: (1) engineering and characterization of the desired fluorescent reporter protein, (2) cloning of the reporter protein into the Thy1 vector, (3) linearization and purification of the new Thy1 const...    »
 
Journal title abbreviation:
Cold Spring Harb Protoc 
Year:
2015 
Journal volume:
2015 
Journal issue:
10 
Pages contribution:
937-40 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
1940-3402 
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)