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Document type:
Zeitschriftenaufsatz 
Author(s):
Fraga García, P.; Freiherr von Roman, M.; Reinlein, S.; Wolf, M.; Berensmeier, S. 
Title:
Impact of Nanoparticle Aggregation on Protein Recovery through a Pentadentate Chelate Ligand on Magnetic Carriers 
Abstract:
The growing need for more efficient separation techniques still dominates downstream processing of biomolecules, thus encouraging the continuous development of advanced anomaterials. In this paper we present an improved process for recovering recombinant histidine tagged green fluorescent protein from an E. coli cell lysate. Superparamagnetic ore−shell nanocarriers are functionalized with a pentadentate chelate affinity ligand and then loaded with metal ions (Cu2+, Ni2+, or Zn2+). The separatio...    »
 
Keywords:
protein recovery; biomolecule purification; magnetic separation; pentadentate chelate ligand; aggregation; agglomeration; nanoparticles 
Journal title:
ACS Applied Materials & Interfaces 
Year:
2014 
Year / month:
2014-06 
Language:
en 
Fulltext / DOI:
TUM Institution:
Fachgebiet für Selektive Trenntechnik