User: Guest  Login
Document type:
Zeitschriftenaufsatz 
Author(s):
Lisak, K.; Toro-Sierra, J.; Kulozik, U.; Božanić, R.; Cheison, S. C. 
Title:
Chymotrypsin selectively digests β-lactoglobulin in whey protein isolate away from enzyme optimal conditions: potential for native α-lactalbumin purification 
Abstract:
The present study examines the resistance of the α-lactalbumin to α-chymotrypsin (EC 3.4.21.1) digestion under various experimental conditions. Whey protein isolate (WPI) was hydrolysed using randomised hydrolysis conditions (5 and 10% of WPI; pH 7.0, 7.8 and 8.5; temperature 25, 37 and 50 °C; enzyme-to-substrate ratio, E/S, of 0.1%, 0.5 and 1%). Reversed-phase high performance liquid chromatography (RP-HPLC) was used to analyse residual proteins. Heat, pH adjustment and two inhibitors (Bowma...    »
 
Keywords:
Animals; Chromatography, High Pressure Liquid; Chymotrypsin/metabolism; Hot Temperature; Hydrogen-Ion Concentration; hydrolysis; Lactalbumin/isolation {&} purification; Lactoglobulins/metabolism; Milk; Milk Proteins/chemistry; Protease Inhibitors/pharmacology; Substrate Specificity; Whey proteins 
Journal title:
The Journal of Dairy Research 
Year:
2013 
Journal volume:
80 
Journal issue:
Pages contribution:
14--20