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Document type:
Zeitschriftenaufsatz 
Author(s):
Brunner, Vincent; Hussein, Mohamed; Becker, Thomas 
Title:
Biomass estimation in Pichia pastoris cultures by combined single-wavelength fluorescence measurements 
Abstract:
In this work, the evolution of different biogenic fluorophores involved in the metabolism of Pichia pastoris was determined at four different single-wavelength pairs (excitation/emission) during batch culture in microwell plates and used for effective and reliable biomass estimation by means of chemometric tools. The chemometric tools for biomass estimation were multiple linear regression (MLR), partial least squares regression (PLSR), and principal component regression (PCR). Variable importanc...    »
 
Keywords:
Pichia pastoris bioprocess monitoring biomass estimation fluorescence spectroscopy partial least squares regression principal component regression 
Journal title:
Biotechnology and Bioengineering 
Year:
2016 
Journal volume:
113 
Journal issue:
11 
Pages contribution:
2394-2402 
Print-ISSN:
1097-0290