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Dokumenttyp:
journal article 
Autor(en):
Guo, Shihui; Skala, Wolfgang; Magdolen, Viktor; Briza, Peter; Biniossek, Martin L; Schilling, Oliver; Kellermann, Josef; Brandstetter, Hans; Goettig, Peter 
Titel:
A Single Glycan at the 99-Loop of Human Kallikrein-related Peptidase 2 Regulates Activation and Enzymatic Activity. 
Abstract:
Human kallikrein-related peptidase 2 (KLK2) is a key serine protease in semen liquefaction and prostate cancer together with KLK3/prostate-specific antigen. In order to decipher the function of its potential N-glycosylation site, we produced pro-KLK2 in Leishmania tarentolae cells and compared it with its non-glycosylated counterpart from Escherichia coli expression. Mass spectrometry revealed that Asn-95 carries a core glycan, consisting of two GlcNAc and three hexoses. Autocatalytic activation...    »
 
Zeitschriftentitel:
J Biol Chem 
Jahr:
2016 
Band / Volume:
291 
Heft / Issue:
Seitenangaben Beitrag:
593-604 
Sprache:
eng 
Print-ISSN:
0021-9258 
TUM Einrichtung:
Frauenklinik und Poliklinik