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Dokumenttyp:
Zeitschriftenaufsatz 
Autor(en):
Author(s): Angelika E. Schnieke, Alexander J. Kind, William A. Ritchie, Karen Mycock, Angela R. Scott, Marjorie Ritchie, Ian Wilmut, Alan Colman, Keith H. S. Campbell 
Titel:
Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts 
Abstract:
Abstract Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from t...    »
 
Zeitschriftentitel:
Science (New York, N.Y.) 
Jahr:
1997 
Band / Volume:
278 (5346) 
Seitenangaben Beitrag:
2130-3