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- Original title:
- Structural and functional evolution of the alternative splicing factor LS2 from Drosophila melanogaster
- Translated title:
- Strukturelle und funktionelle Evolution des alternativen Spleißfaktors LS2 von Drosophila melanogaster
- Author:
- Kawale, Ashish
- Year:
- 2017
- Document type:
- Dissertation
- Faculty/School:
- Fakultät für Chemie
- Advisor:
- Sattler, Michael (Prof. Dr.)
- Referee:
- Sattler, Michael (Prof. Dr.); Niessing, Dierk (Prof. Dr.)
- Language:
- en
- Subject group:
- CHE Chemie
- Keywords:
- LS2, G-quadruplex, U2AF50, RRM
- Translated keywords:
- LS2, G-quadruplex, U2AF50, RRM
- TUM classification:
- CHE 808d; CHE 244d
- Abstract:
- This thesis employs an integrated structural biology approach to characterize the RNA binding of the alternative splicing factor LS2, a paralog of the essential splicing factor U2AF50 in Drosophila melanogaster. The LS2 RNA binding domains (RRMs) have a conserved RRM fold but exhibit specific features to interact with G-quadruplex RNA in target messenger RNAs, distinct from U2AF50, which recognizes the single-stranded pyrimidine-rich RNA.
- Translated abstract:
- Diese Arbeit beschäftigt sich mit einem integrierten Ansatz der Strukturbiologie zur Charakterisierung der RNA-Bindung des alternativen Spleißfaktors LS2, eines Paralogs des essentiellen Spleißfaktors U2AF50 in Drosophila melanogaster. Die LS2-RNS-Bindungsdomänen (RRMs) weisen eine konservierte RRM-Faltung auf, zeigen aber spezifische Eigenschaften, um mit G-Quadruplexen in Ziel-Messenger-RNS zu interagieren, im Gegensatz zu U2AF50, was einzelsträngige Pyrimidin-reiche RNS erkennt.
- WWW:
- https://mediatum.ub.tum.de/?id=1342237
- Date of submission:
- 10.01.2017
- Oral examination:
- 13.02.2017
- File size:
- 12968029 bytes
- Pages:
- 135
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20170213-1342237-1-6
- Last change:
- 13.02.2018
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