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Dokumenttyp:
journal article 
Autor(en):
Hoffmann, D; Assfalg-Machleidt, I; Nitschko, H; von der Helm, K; Koszinowski, U; Machleidt, W 
Titel:
Rapid enzymatic test for phenotypic HIV protease drug resistance. 
Abstract:
A phenotypic resistance test based on recombinant expression of the active HIV protease in E. coli from patient blood samples was developed. The protease is purified in a rapid one-step procedure as active enzyme and tested for inhibition by five selected synthetic inhibitors (amprenavir, indinavir, nelfinavir, ritonavir, and saquinavir) used presently for chemotherapy of HIV-infected patients. The HPLC system used in a previous approach was replaced by a continuous fluorogenic assay suitable fo...    »
 
Zeitschriftentitel:
Biol Chem 
Jahr:
2003 
Band / Volume:
384 
Heft / Issue:
Seitenangaben Beitrag:
1109-17 
Sprache:
eng 
Print-ISSN:
1431-6730 
TUM Einrichtung:
r Virologie