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Document type:
journal article 
Author(s):
Durand, GM; Marandi, N; Herberger, SD; Blum, R; Konnerth, A 
Title:
Quantitative single-cell RT-PCR and Ca(2+) imaging in brain slices. 
Abstract:
We have established a quantitative reverse transcriptase-PCR (RT-PCR) approach for the analysis of RNA transcript levels in individual cells of living brain slices. Quantification is achieved by using rapid-cycle, real-time PCR protocols and high-resolution external cDNA standard curves for the gene of interest. The method consists of several procedures, including cell soma harvest, reverse transcription, and an optimized cDNA purification step, which allowed us to quantify transcripts in small...    »
 
Journal title abbreviation:
Pflugers Arch 
Year:
2006 
Journal volume:
451 
Journal issue:
Pages contribution:
716-726 
Language:
eng 
Print-ISSN:
0031-6768 
TUM Institution:
r Neurowissenschaften