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Document type:
journal article 
Author(s):
Stroh, A; Tsai, HC; Wang, LP; Zhang, F; Kressel, J; Aravanis, A; Santhanam, N; Deisseroth, K; Konnerth, A; Schneider, MB 
Title:
Tracking stem cell differentiation in the setting of automated optogenetic stimulation. 
Abstract:
Membrane depolarization has been shown to play an important role in the neural differentiation of stem cells and in the survival and function of mature neurons. Here, we introduce a microbial opsin into ESCs and develop optogenetic technology for stem cell engineering applications, with an automated system for noninvasive modulation of ESC differentiation employing fast optogenetic control of ion flux. Mouse ESCs were stably transduced with channelrhodopsin-2 (ChR2)-yellow fluorescent protein an...    »
 
Journal title abbreviation:
Stem Cells 
Year:
2011 
Journal volume:
29 
Journal issue:
Pages contribution:
78-88 
Language:
eng 
Print-ISSN:
1066-5099 
TUM Institution:
Abteilung für Neuroradiologie ; Institut für Neurowissenschaften