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Document type:
journal article 
Author(s):
Milosevic, S; Behrends, U; Christoph, H; Mautner, J 
Title:
Direct mapping of MHC class II epitopes. 
Abstract:
Despite technical improvements, the mapping of MHC class II epitopes within complex antigens by genetic or biochemical methods is still laborious and expensive. Here, we describe a simple and fast procedure to directly map T helper cell epitopes within known antigens by bacterial expression cloning. Short antigenic fragments, created by digestion of the coding sequence of the antigen with frequently cutting restriction enzymes, are randomly ligated to the coding sequence of GFP in a bacterial ex...    »
 
Journal title abbreviation:
J Immunol Methods 
Year:
2005 
Journal volume:
306 
Journal issue:
1-2 
Pages contribution:
28-39 
Language:
eng 
Print-ISSN:
0022-1759 
TUM Institution:
Kinderklinik und Poliklinik