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Dokumenttyp:
journal article 
Autor(en):
Busch, R; Cesar, D; Higuera-Alhino, D; Gee, T; Hellerstein, MK; McCune, JM 
Titel:
Isolation of peripheral blood CD4(+) T cells using RosetteSep and MACS for studies of DNA turnover by deuterium labeling. 
Abstract:
Tracking deuterium ((2)H) incorporation into cellular DNA, after administration of (2)H(2)O or (2)H(2)-glucose, is a recently developed, broadly applicable method for measuring in vivo cell proliferation and turnover that can be used safely in humans. This approach has been used to evaluate the turnover of T-cell subpopulations purified from the peripheral blood of HIV-1-infected patients using fluorescence-activated cell sorting (FACS). A requirement for widespread adoption of this approach for...    »
 
Zeitschriftentitel:
J Immunol Methods 
Jahr:
2004 
Band / Volume:
286 
Heft / Issue:
1-2 
Seitenangaben Beitrag:
97-109 
Sprache:
eng 
Print-ISSN:
0022-1759 
TUM Einrichtung:
r Medizinische Statistik und Epidemiologie