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Dokumenttyp:
journal article 
Autor(en):
Lehle, K; Hoenicka, M; Jacobs, VR; Schmid, FX; Birnbaum, DE 
Titel:
Cryopreservation of human endothelial cells for vascular tissue engineering. 
Abstract:
To investigate the influence of cryopreservation on endothelial cell growth, morphology, and function human umbilical vein endothelial cells (HUVECs) were frozen following a standard protocol. Cell suspensions were exposed to 10% dimethyl sulfoxide in a high-potassium solution, cooled to -80 degrees C at 1 degrees C/min and stored in liquid nitrogen for 7-36 days. Samples were thawed in a 37 degrees C water bath and the cryoprotectant was removed by serial dilution. The growth of cell suspension...    »
 
Zeitschriftentitel:
Cryobiology 
Jahr:
2005 
Band / Volume:
50 
Heft / Issue:
Seitenangaben Beitrag:
154-61 
Sprache:
eng 
Print-ISSN:
0011-2240 
TUM Einrichtung:
Frauenklinik und Poliklinik