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Dokumenttyp:
journal article 
Autor(en):
Lassmann, S; Bauer, M; Rosenberg, R; Nekarda, H; Soong, R; Rüger, R; Höfler, H; Werner, M 
Titel:
Identification of occult tumor cells in node negative lymph nodes of colorectal cancer patients by cytokeratin 20 gene and protein expression. 
Abstract:
BACKGROUND AND AIMS: Evaluation of cytokeratin 20 (CK20) specific quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) and immunohistochemistry (IHC) for detection of occult tumor cells in lymph nodes of 72 patients with colorectal carcinoma (UICC stage I and II). METHODS: Serial sections of formalin-fixed, paraffin-embedded lymph nodes (mean 14.3/case) were used for microdissection, RNA isolation and QRT-PCR and for CK20 IHC using routine protocols. Results of QRT-PCR and IHC...    »
 
Zeitschriftentitel:
Int J Colorectal Dis 
Jahr:
2004 
Band / Volume:
19 
Heft / Issue:
Seitenangaben Beitrag:
87-94 
Sprache:
eng 
Print-ISSN:
0179-1958 
TUM Einrichtung:
r Allgemeine Pathologie und pathologische Anatomie; Chirurgiesche Klinik und Poliklinik