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Document type:
journal article 
Author(s):
Tischbirek, Carsten; Birkner, Antje; Jia, Hongbo; Sakmann, Bert; Konnerth, Arthur 
Title:
Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator. 
Abstract:
In vivo Ca2+ imaging of neuronal populations in deep cortical layers has remained a major challenge, as the recording depth of two-photon microscopy is limited because of the scattering and absorption of photons in brain tissue. A possible strategy to increase the imaging depth is the use of red-shifted fluorescent dyes, as scattering of photons is reduced at long wavelengths. Here, we tested the red-shifted fluorescent Ca2+ indicator Cal-590 for deep tissue experiments in the mouse cortex in vi...    »
 
Journal title abbreviation:
Proc Natl Acad Sci U S A 
Year:
2015 
Journal volume:
112 
Journal issue:
36 
Pages contribution:
11377-82 
Language:
eng 
Print-ISSN:
0027-8424 
TUM Institution:
Institut für Neurowissenschaften