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Dokumenttyp:
journal article 
Autor(en):
von Bomhard, Achim; Elsässer, Alexander; Ritschl, Lucas Maximilian; Schwarz, Silke; Rotter, Nicole 
Titel:
Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media - Vitrification versus Slow Freezing Methods. 
Abstract:
Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PG), and glycerol (GLY), and two media, namely Dulbecco's modified Eagle medium...    »
 
Zeitschriftentitel:
PLoS ONE 
Jahr:
2016 
Band / Volume:
11 
Heft / Issue:
Seitenangaben Beitrag:
e0149660 
Sprache:
eng 
TUM Einrichtung:
Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie