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Dokumenttyp:
journal article 
Autor(en):
Reichert, Maximilian; Rhim, Andrew D; Rustgi, Anil K 
Titel:
Culturing primary mouse pancreatic ductal cells. 
Abstract:
The most common subtype of pancreatic cancer is pancreatic ductal adenocarcinoma (PDAC). PDAC resembles ductal cells morphologically. To study pancreatic ductal cell (PDC) and pancreatic intraepithelial neoplasia (PanIN)/PDAC biology, it is essential to have reliable in vitro culture conditions. Here we describe a methodology to isolate, culture, and passage PDCs and duct-like cells from the mouse pancreas. It can be used to isolate cells from genetically engineered mouse models (GEMMs), providi...    »
 
Zeitschriftentitel:
Cold Spring Harb Protoc 
Jahr:
2015 
Band / Volume:
2015 
Heft / Issue:
Seitenangaben Beitrag:
558-61 
Sprache:
eng 
Print-ISSN:
1940-3402 
TUM Einrichtung:
II. Medizinische Klinik und Poliklinik