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Dokumenttyp:
journal article 
Autor(en):
Weber, Julia; Öllinger, Rupert; Friedrich, Mathias; Ehmer, Ursula; Barenboim, Maxim; Steiger, Katja; Heid, Irina; Mueller, Sebastian; Maresch, Roman; Engleitner, Thomas; Gross, Nina; Geumann, Ulf; Fu, Beiyuan; Segler, Angela; Yuan, Detian; Lange, Sebastian; Strong, Alexander; de la Rosa, Jorge; Esposito, Irene; Liu, Pentao; Cadiñanos, Juan; Vassiliou, George S; Schmid, Roland M; Schneider, Günter; Unger, Kristian; Yang, Fengtang; Braren, Rickmer; Heikenwälder, Mathias; Varela, Ignacio; Saur, Die...    »
 
Titel:
CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice. 
Abstract:
Here, we show CRISPR/Cas9-based targeted somatic multiplex-mutagenesis and its application for high-throughput analysis of gene function in mice. Using hepatic single guide RNA (sgRNA) delivery, we targeted large gene sets to induce hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). We observed Darwinian selection of target genes, which suppress tumorigenesis in the respective cellular/tissue context, such as Pten or Cdkn2a, and conversely found low frequency of Brca1/2 al...    »
 
Zeitschriftentitel:
Proc Natl Acad Sci U S A 
Jahr:
2015 
Band / Volume:
112 
Heft / Issue:
45 
Seitenangaben Beitrag:
13982-7 
Sprache:
eng 
Print-ISSN:
0027-8424 
TUM Einrichtung:
II. Medizinische Klinik und Poliklinik; Institut für Allgemeine Pathologie und pathologische Anatomie; Institut für Virologie; Institut für Radiologie