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Dokumenttyp:
journal article 
Autor(en):
Lahm, Harald; Doppler, Stefanie; Dreßen, Martina; Werner, Astrid; Adamczyk, Klaudia; Schrambke, Dominic; Brade, Thomas; Laugwitz, Karl-Ludwig; Deutsch, Marcus-André; Schiemann, Matthias; Lange, Rüdiger; Moretti, Alessandra; Krane, Markus 
Titel:
Live fluorescent RNA-based detection of pluripotency gene expression in embryonic and induced pluripotent stem cells of different species. 
Abstract:
The generation of induced pluripotent stem (iPS) cells has successfully been achieved in many species. However, the identification of truly reprogrammed iPS cells still remains laborious and the detection of pluripotency markers requires fixation of cells in most cases. Here, we report an approach with nanoparticles carrying Cy3-labeled sense oligonucleotide reporter strands coupled to gold-particles. These molecules are directly added to cultured cells without any manipulation and gene expressi...    »
 
Zeitschriftentitel:
Stem Cells 
Jahr:
2015 
Band / Volume:
33 
Heft / Issue:
Seitenangaben Beitrag:
392-402 
Sprache:
eng 
Print-ISSN:
1066-5099 
TUM Einrichtung:
I. Medizinische Klinik und Poliklinik; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene; Lehrstuhl für Regenerative Medizin bei kardiovaskulären Erkrankungen